84-1103-89　Rap1 (hRap1, Telomeric Repeat Binding Factor 2 Interacting Protein, TERF2IP, TRF2 Interacting Telomeric Protein RAP1) 100ug　R1120-13A

特徴

• Human Rap1 was the first identified ortholog of the yeast telomeric protein scRap1, which shares 3 conserved sequence motifs in common.
• HRap1 is located at telomeres and affects telomere length by being recruited to telomeres by TRF2 instead of directly binding to the telomere like scRap1.
• The hRap1 cDNA codes for a protein of 399 amino acids with approximate molecular weight of 47kD.
• The hRap1 protein does not interact with TRF1.
• TRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length.
• TRF2, like TRF1 contains a Myb-related DNA binding motif.
• It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes.
• TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini.
• The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells.
• Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types.
• Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry/Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Western Blot: 2ug/mlImmunofluorescence (IC): 2-10ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.