Rad50, a 153kD protein involved in DNA double strand break repair, is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process.
Upon DNA damage, Rad50 and Mre11 redistribute and are colocalized within the nucleus of damaged cells.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Not suitable for Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Immunoprecipitation: 3-4x10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.02% sodium azide.
Specificity：Recognizes mouse Rad50 at ~153kD. Species Crossreactivity: human and hamster.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hm Hu Mo
Immunogen：Synthetic peptide corresponding to the C-terminal region (604aa) of the mouse Rad50 protein. Cellular Localization: Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.
