84-1103-79　［取扱停止］SP19 (pan Sodium Channel) 200ul　S5368

特徴

• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:200 using ECL on rat brain membranesImmunohistochemistry: Frozen mouse brain sections; Paraffin mouse spinal cord sections
• Note: All procedures that are going to receive a full-length protein should be performed at 4°C, and the following protease inhibitor mixture should be used: pepstatin A (1ug/ml), leupeptin (1ug/ml), aprotinin (1ug/ml), Pefabloc SC (0.2mM), benzamidine (0.1mg/ml), and calpain inhibitors I and II (8ug/ml each)
• Optimal dilutions to be determined by the researcher.
• Included Negative Control:S5368A: SP19 (pan Sodium Channel), Rat, Control PeptideSupplied as a lyophilized powder.
• Reconstitute in 100ul sterile ddH2O.
• Preincubate 1ug of peptide with 1ug of antibody for one hour at RT.
• Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRbI1904-46Q: IgG, H&L (HRP) Pab Gt xRb
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months after receipt at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.