Voltage-gated sodium channels (Nav) are essential for the generation of action potentials and for cell excitability.
Nav channels are activated in response to depolarization and selectively allow flow of Na+ ions.
To date, nine Nav a subunits have been cloned and named Nav1.1-Nav1.9.
The Nav channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (Nav1.1, Nav1.2, Nav1.3, Nav1.4, Nav1.6 and Nav1.7) and TTX-resistant (Nav1.5, Nav1.8 and Nav1.9) Mammalian sodium channels are heterotrimers, composed of a central, pore-forming a subunit and two auxiliary b subunits.
The expression of the a subunit isoforms is developmentally regulated and tissue specific.
Sodium channels in the adult central nervous system and heart contain b1 through b4 subunits, whereas sodium channels in adult skeletal muscle have only the b1 subunit.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot (Rat brain membranes): 1:200Immunohistochemistry: Rat brain sections
Optimal dilutions to be determined by the researcher.
Control Peptide:N0530-05P Nav1.1 Control Antigen (Brain Type I Sodium Channel, Scn1a, BI),
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 1% BSA, 5% sucrose, 0.025% sodium azide.
Specificity：Recognizes rat Nav1.1. Species crossreactivity: mouse. Does not cross react with any other sodium channel antigens tested so far.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide corresponding to aa465-481 (KY)TAS EHSRE PSAAG RLSD of rat Nav1.1 (Accession P04774). Epitope Location: Intracellular loop between I and II domains. Species sequence homology: human.
