Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it.
Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages.
The inward rectification is mainly due to the blockage of outward current by internal magnesium.
Can be blocked by extracellular barium.
Can form cardiac and smooth muscle-type KATP channels with ABCC9.
KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation (By similarity).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200.
(Rat pancreas membrane)Optimal dilution determined by the researcher.
Control Antigen Included:K1870A: Preadsorption Control: 2ug peptide/1ug antibody.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.025% sodium azide.
Specificity：Recognizes rat Kir6.2. Does not crossreact with Kir6.1.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to aa372-385,(C)SVAVAKAKPKFSIS of rat Kir6.2 (Accession number P70673). Epitope location: Intracellular, C-terminal part. Species sequence homology: mouse-100%; human- 12/14.
