Ran is a small GTPase of the Ras family that plays a central role in the spacial and temperal organization of eukaryotic cells.
During interphase, Ran-GDP localizes to the cytoplasm and Ran-GTP to the nucleus.
This polarized localization of Ran ensures its role in nuclear transport.
During mitosis, Ran-GTP is chromatin associated, where it promotes spindle assembly and nuclear envolope formation.
In S phase, Ran-GTP associates with and inhibits MCM helicase, ensuring precise chromosomal DNA duplication during the cell cycle.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000.
Incubate membrane with 1X TBS, 5% BSA.
0.1% Tween-20 at 4ºC with gentle shaking overnight
Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human Ran protein. Does not crossreact with other small GTPases of the Ras family. Species Crossreactivity: mouse, rat, monkey, hamster and bovine
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Hm Hu Mk Mo Rt
Immunogen：Two synthetic peptides corresponding to amino acids surrounding Arg29 and Asn143 of human Ran. Species Sequence Homology: D. melanogaster and Xenopus, 100%
