In both prokaryotic and eukarytic cells the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones (1-3).
HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (4).
It is now clear that a significant amount of HSP60 is also present in the cytosol of many cells and that it is induced by stress, inflammatory and immune responses, autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes (5-8).
Applications:Suitable for use in Western Blot, Immunofluorescence and Flow Cytometry.
Other applications have not been tested.
Recommended Dilutions: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunofluorescence (IF-IC): 1:50 Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human HSP60 protein. Does not cross-react with other HSPs. Species crossreactivity: mouse, rat, monkey, D. melanogaster.
Isotype：IgG
Calc Applications Abbrev：FC IF WB
Calc Crossreactivity：Dr Hu Mk Mo Rt
Immunogen：Synthetic peptide surrounding Asp85 of human HSP60.
