HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha.
Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen.
HIF is a transcriptional complex that plays a critical role in oxygen homeostasis.
HPH is an essential component of the pathway through which cells sense oxygen.
In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction.
Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.
HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins.
It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha.
It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.
Positive Control:Mouse kidney lysate
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Tris-citrate/phosphate [pH 7-8]
Specificity：Specific for PHD2. Species Crossreactivity: Reacts with mouse PHD2 protein. The immunogen used for this antibody production has 100% homology with rat protein. There is no significant homology with the human protein.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Mo Rt
Immunogen：A synthetic peptide made to an internal portion of the mouse PHD2 protein sequence (between residues 300-400).
Shelf Life：1year
EU Commodity Code：30021010
商品名：PHD2 (Prolyl Hydroxylase Domain Containing Protein 2, PHD-2, Chromosome 1 Open Reading Frame 12, C1orf12, DKFZp761F179, ECYT3, EGL-9 Homolog of C. elegans 1, EGL Nine (C. elegans) Homolog 1, Egl Nine Homolog 1, EGLN1, HIF Prolyl Hydroxylase 2, HIF-PH2, HIFPH2, Hypoxia Inducible Factor Prolyl Hydroxylase 2, HPH2, HPH-2, ORF13, P4H2, PNAS-118, PNAS-137, Rat Homolog of SM20, SM20, SM-20, Zinc Finger MYND Domain Containing Protein 6, ZMYND6) 100ul
