HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha.
Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen.
HIF is a transcriptional complex that plays a critical role in oxygen homeostasis.
HPH is an essential component of the pathway through which cells sense oxygen.
In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction.
Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.
HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins.
It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha.
It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.
Applications:Suitable for use in Western Blot, Flow Cytometry, Immunoprecipitation and Immunohistochemistry..
Detects a band at 46-50kDOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C.
For long-term storage, aliquot and store at 4°C.
Do not freeze.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.09% sodium azide.
Specificity：Recognizes human PHD2. Recognizes an epitope mapped to a region between residues 1 and 50 of human PHD2 (NP_071334.1) (GeneID 54583). Does not react with mouse.
Isotype：IgG
Calc Applications Abbrev：FC IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the C-terminal portion of human PHD2/HIF Prolyl Hydroxylase 2 (between amino acids 350-426).
Shelf Life：1year
Swiss Prot Number：Q9GZT9
EU Commodity Code：30021010
商品名：PHD2 (Prolyl Hydroxylase Domain Containing Protein 2, PHD-2, Chromosome 1 Open Reading Frame 12, C1orf12, ECYT3, EGL-9 Homolog of C. elegans 1, EGL Nine (C. elegans) Homolog 1, Egl Nine Homolog 1, EGLN1, HIF Prolyl Hydroxylase 2, HIF-PH2, HIFPH2, Hypoxia Inducible Factor Prolyl Hydroxylase 2, HPH2, HPH-2, ORF13, P4H2, PNAS-118, PNAS-137, Rat Homolog of SM20, SM20, SM-20, Zinc Finger MYND Domain Containing Protein 6, ZMYND6) 100ul
