HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha.
Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen.
HIF is a transcriptional complex that plays a critical role in oxygen homeostasis.
HPH is an essential component of the pathway through which cells sense oxygen.
In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction.
Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis
HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins.
It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha.
It targets HIF through the hydroxylation for proteasomal degredation via the von Hippel-Lindau ubiquitylation complex.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：As reported
Specificity：This antibody is specific for human HIF prolyl hydroxylase 2. Species Crossreactivity: Human. This antibody does not appear to work in mouse. Results have been mixed in rat with success in western analysis and immunofluorescence on rat endothelial cells and negative results with PC12 cells. Other species have not been tested.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide made to an N-terminal sequence of the human EGL9 homolog 1 (between residues 1-50). [LocusLink ID 54583].
Shelf Life：1year
Swiss Prot Number：Q9GZT9
EU Commodity Code：30021010
商品名：PHD2 (Prolyl Hydroxylase Domain Containing Protein 2, PHD-2, Chromosome 1 Open Reading Frame 12, C1orf12, DKFZp761F179, ECYT3, EGL-9 Homolog of C. elegans 1, EGL Nine (C. elegans) Homolog 1, Egl Nine Homolog 1, EGLN1, HIF Prolyl Hydroxylase 2, HIF-PH2, HIFPH2, Hypoxia Inducible Factor Prolyl Hydroxylase 2, HPH2, HPH-2, ORF13, P4H2, PNAS-118, PNAS-137, Rat Homolog of SM20, SM20, SM-20, Zinc Finger MYND Domain Containing Protein 6, ZMYND6) 100ug
