53BP1 binds to the tumor suppressor protein p53 and has a potential role in DNA damage responses.
Small interfering RNA (siRNA) directed against 53BP1 in mammalian cells to demonstrated that 53BP1 is a key transducer of the DNA damage checkpoint signal.
53BP1 was required for p53 accumulation, G2-M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation.
53BP1 played a partially redundant role in phosphorylation of the downstream checkpoint effector proteins Brca1 and Chk2 but was required for the formation of Brca1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: a band is seen at ~220kD
Immunofluorescence: 1:500.
Immunofluorescent staining of mouse B cells.
The cells were first fixed with Formaldehyde and permeabilized using Triton-X100.
The 53BP1 antibody was incubated for one hour at room temperature in PBS + 0.2% BSA + 0.1% Tween 20.
The secondary antibody used was an Alexa fluor 633 goat anti-rabbit used at a dilution of 1:500, and was incubated at RT for one hour in PBS, 0.2% BSA, 0.1% Tween 20
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Tris-citrate/phosphate, pH 7.2.
Specificity：Specific for human 53BP1 protein. Species Crossreactivity: Reacts with human and mouse proteins. Other species have not been tested.
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Hu Mo
Immunogen：A synthetic peptide, which represents a portion of human tumor protein p53 binding protein 1 between residues 350 and 400 using the numbering given in entry NP_005648.1.
