FPRL1 is a low affinity receptor to N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors.
Binding of FMLP to the receptor causes activation of neutrophils.
This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system.
The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4).
FPRL1 has been reported in phagocytes, monocytes, neutrophils, differentiated myeloid cells from bone marrow, granulocyte HL-60 cells, and synovial fibroblasts.
ESTs have been isolated from blood, leukocyte, lung, and placenta libraries.
Applications:Suitable for use in Immunofluorescence, Flow Cytometry and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunofluorescence: 1:400-1:500Flow Cytometry: 1:100 (using HL-60 as positive control)Western Blot: 1:4000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2.
Specificity：Recognizes human Formyl Peptide Receptor-Like 1 (FPRL1) at ~38kD.
Isotype：IgG
Calc Applications Abbrev：FC IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to ASWGGTPEERLKC on the 2nd extracellular loop of human FPRL1 conjugated to KLH.
