Numb is an evolutionarily conserved membrane-associated protein.
It exists in four isoforms that differ in two functional domains: a phosphotyrosine-binding (PTB) domain and a proline-rich region (PRR).
It plays a role in the control of cell fate decisions during development and antagonizes the actions of Notch.
Numb proteins appear to control the self-renewal of all neural progenitor cells and could possibly play similar roles in stem-cell maintenance in other tissues.
Cellular Localization: Membrane-associated
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Tris-glycine and 150 mM NaCl
Specificity：This antibody is specific for all four isoforms of the Numb protein. Species Crossreactivity: This antibody reacts with human protein, and is expected to react with mouse and chicken based on sequence homology. Other species have not been tested.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：A synthetic peptide made to a C-terminal region of the murine numb protein sequence (between residues 600-653). Homology: The immunogen used for this antibody production has 100% homology with mouse, human and chicken (isoforms 1-4) proteins and 90% homology with rat numb protein (isoforms unknown).
