NuMA (nuclear mitotic apparatus) is a long coil-coiled protein that plays a role in the interphase and mitosis phases of a cell cycle.
In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions.
During mitosis, it is associated with spindle microtubule organization and chromosome positioning.
Therefore, this antibody is useful as a cell cycle marker.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:500Western Blot: 1:1,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Whole antisera
Form：Supplied as a liquid with 0.09% sodium azide.
Specificity：Recognizes human NuMA. at 220kD. Species Crossreactivity: Reactive to most mammalian species tested.
Isotype：IgG
Calc Applications Abbrev：IF IP WB
Calc Crossreactivity：Hu
Immunogen：Full-length recombinant human NuMA expressed in insect Sf9 cells using baculovirus expression system, then purified to near homogeneity prior to immunization.
