84-1102-46　［取扱停止］Nucleolin (NCL, Nucl, C23, FLJ45706, Protein C23) 100ul　N6945-01E

特徴

• Nucleolin is an abundant, 106kD nucleolar phosphoprotein.
• It is the major protein in actively dividing cells, whereas the degraded forms are relatively abundant in non-dividing cells.
• Stability of the nucleolin protein is cell proliferation-dependent.
• It plays a role in rDNA transcription, organization, and rRNA processing.
• It may alter DNA organization in response to cell cycle controls.
• It is also a good mammalian nucleolus marker.
• Cellular Localization: Nucleoli
• Positive Control: PD31 murine pre-B cells for immunofluorescence and crude PD31 nuclear extracts for Western Blot.
• Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (paraffin-embedded sections).
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50%glycerol, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.