Nerve Growth Factor is the first of a series of neurotrophic factors that were found to influence the growth and differentiation of sympathetic and sensory neurons.
It is comprised of alpha, beta, and gamma subunits.
The beta subunit is responsible for its growth stimulating activity.
The synthesis of NGF in astrocytes is enhanced by various other cytokines: IL-1, TNF-alpha, PDGF and TGF-beta.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Inhibition.
Other applications not tested.
Recommended Dilution:ELISA: 1-3ug/mlWestern Blot: 1-3ug/mlImmunohistochemistry: 1-3ug/mlInhibition of Biological Activity In vitro: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder. Reconstitute in 500ul sterile ddH2O.
Specificity：Recognizes mouse beta Nreve Growth Factor. A crossreactivity of <1% has been shown to recombinant human BDNF, NT3, NT4/5 by ELISA. Completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Species Crossreactivity: Rat, human and avian. Does not crossreact with bovine.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Av Hu Mo Rt
Immunogen：Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE).
