Mammalian MD-1 is a homolog of Chicken MD-1, which was previously isolated as a v-myb-regulated gene, since its transcription increases rapidly after v-myb induction.
MD-1 is associated at the cell surface with RP105.
RP105 is a leucine-rich repaeat (LRR) molecule that is expressed on B-lymphocytes.
It was first identified by a mAb that protects spleen B cells from irradiation-induced apoptosis (2).
LRR proteins, such as Toll receptors, have a role in innate immunity.
MD-1 does not have any homology to other molecules.
MD-1 when expressed alone behaves as a secretory protein.
Expression of MD-1 in a cell increases the expression of RP105.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 2ug/ml, in Ramos, a 25kD band is observedImmunohistochemistry: ParaffinOptimal dilutions to be determined by the researcher.
Positive Control:Ramos
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity：Recognizes human MD-1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated synthetic peptide corresponding to aa112-125 of human MD-1.
