Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells.
They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit.
Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5.
MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b.
A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels.
Antibodies to MAP2 are excellent markers on neuronal cells, their perikarya and neuronal dendrites.
In contrast t (tau) is found predominantly in neuronal axons
Dendrite specific MAP is found specifically in the dendritic branching of neurons.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50%glycerol, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：chicken
Source Antibody：human
Grade：Serum
Purity：Whole antisera
Form：As reported
Specificity：Species Crossreactivity: This antibody reacts with human, rat, mouse and bovine protein. Other species have not been tested.
Isotype：IgY
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Mix of recombinant human construct of projection domain sequences, amino acids 377-1505: Prot-r-MAP2-P1, Prot-r-MAP2-P2 and Prot-r-MAP2-P3.
