Probably involved in formation of autophagosomal vacuoles (autophagosomes).
The subunit is 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 1:100-1:1000Western Blot: 1:100-1:1000Immunofluorescence: 1:100-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Serum
Purity:Serum
Form:Supplied as a lyophilized powder. Reconstitute with 100ul of sterile ddH2O.
Specificity:Recognizes human MAP1LC3C. Does not recognize forms MAP1LC3A and MAP1LC3B.
Isotype:IgG
Calc Applications Abbrev:IF IHC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to CQEEVAGIRAKF at the N-terminal of human MAP1LC3 C conjugated to Blue carrier Protein. Peptide Sequence Homology: monkey (Macaca mulatta) and canine.