MAP1A and MAP1B are microtubule-associated protein which mediate the physical interactions between microtubules and components of the cytoskeleton (probably involved in autophagosome formation).
MAP1A and MAP1B each consist of a heavy chain subunit and 3 different light chain subunits (LC1, LC2 and LC3).
MAP1LC3A is one of the light chain subunits and can associate with either MAP1A or MAP1B.
The precursor form of MAP1LC3A is cleaved by APG4/ATG4B to form the cytosolic form LC3-1.
This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
MAP1LC3A is most abundant in heart, brain, liver, skeletal muscle and testis but is absent in thymus and peripheral leukocytes.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:100-1:1000Immunohistochemistry: 1:100-1:1000Immunofluorescence: 1:100-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 40% glycerol.
Specificity：Recognizes human MAP1LC3A. Species Crossreactivity: rat. Species sequence homology: mouse.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to the N-terminal of human MAP1LC3A protein conjugated to Blue Carrier Protein. The sequence is homologous with mouse and rat form of MAP1LC3A.
