Alpha 2 macroglobulin is a serum protein which is mainly synthesized in the liver.
It functions as a broad range irreversible proteinase inhibitor that forms a "trap" around most proteases.
Alpha 2 macroglobulin is is a tetrameric molecule, and with a molecular weight of 720kD, it so large a molecule that it tends to remain intravascular.
Following conformational change, the protinase is deposited within a central cavity where it is still active but prevented from further contact with its substrate.
Removal of the complex is mediated by the generation of recognition sites which react with receptors on a variety of cells including macrophages and liver cells.
Alpha 2 macroglobulin is localized on the surface of peripheral blood lymphocytes.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:80,000-1:400,000Immunohistochemistry (Frozen/Paraffin): 1:10-1:500 Western Blot: 1:2000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide. Reconstitute with 100ul sterile ddH2O.
