Lysyl oxidase (LOX), a copper-containing amine oxidase, belongs to a heterogeneous family of enzymes that oxidize primary amine substrates to reactive aldehydes.
It plays a vital role in the formation and repair of the extracellular matrix.
In addition, LOX is a multifunctional enzyme having diverse biological functions such as developmental regulation, tumor suppression, cell motility, and cellular senescence.
The secreted form of LOX is responsible for the invasive properties of hypoxic human cancer cells.
Thus, it is essential for hypoxia-induced metastasis and is a good therapeutic target for preventing and treating metastases.
Applications:Suitable for use in Immunocytochemistry/Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000.
A band is seen at ~58kD.
May see bands at ~32kD (mature, secreted form), 50kD and 58kD (pro and glycosylated forms) since L3514-50B is a highly glycosylated protein
Immunohistochemistry (Paraffin): 1:200Immunocytochemistry/Immunofluorescence: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS. 0.02% sodium azide.
Specificity：Recognizes human LOX. Species Crossreactivity: mouse, rat, bovine and porcine.
Isotype：IgG
Calc Applications Abbrev：IC IF IHC WB
Calc Crossreactivity：Bo Hu Mo Po Rt
Immunogen：Two synthetic peptides corresponding to aa200-300 from human LOX and aa300-400 also from human LOX. Cellular Localization: Cytosolic, nuclear. Species Sequence Homology (92%): Chicken, zebrafish, xenopus.
