The Lipin family of nuclear proteins contains three members: Lipin-1, Lipin-2 and Lipin-3, all of which contain a nuclear signal sequence, a highly conserved amino-terminal (NLIP) domain and a carboxy-terminal (CLIP) domain.
LPIN1 (Lipin-1) is crucial for normal adipose tissue development and metabolism.
LPIN1 selectively activates a subset of PGC-1á target pathways, including fatty acid oxidation and mitochondrial oxidative phosphorylation by inducing expression of the nuclear receptor PPARá.
LPIN1 also inactivates the lipogenic program and suppresses circulating lipid levels.
An abundance of LPIN1 promotes fat accumulation and insulin sensitivity, whereas a deficiency in LPIN1 may deter normal adipose tissue development, resulting in insulin resistance and lipodystrophy, a heterogeneous group of disorders characterized by loss of body fat, fatty liver, hypertriglyceridemia and insulin resistance.
Cellular Localization: Nuclear
Positive Control: 3T3-L1 cell lysates.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide immunoaffinity chromatography.
Form：PBS + 30% glycerol
Specificity：Species Crossreactivity: This antibody reacts with the mouse protein. Other species have not been tested.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：A synthetic peptide to an internal region (within residues 300-400) of the human LPIN1 protein. [Swiss-Prot# Q14693]. Homology: 100% homology with human protein, 95% with mouse protein, and 90% with rat protein.
