Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation.
During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment.
There are 16 proteins participating in the autophagy pathway in humans.
The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3.
It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression may modify MAP1 microtubule-binding activity during development.
LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes.
LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction.
The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
Applications:Suitable for use in Immunofluorescence, Western Blot Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Whole antisera
Form：Supplied as a liquid.
Specificity：This antibody is specific for the B isoform of LC3. Species Crossreactivity: This antibody reacts with the human and mouse proteins. Other species have not been tested.
Isotype：IgG
Calc Applications Abbrev：IC IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：A synthetic peptide made to the N-terminal region of the human LC3, isoform B protein.
