Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation.
During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment.
There are 16 proteins participating in the autophagy pathway in human.
The autophagy protein LC3, a mammalian homolog of Atg8, was originally identified as microtubule-associated protein 1 light chain 3.
It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development.
LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes.
LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction.
The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 2ug/ml; Bands seen at ~19kD representing LC3-I and ~17kD represent LC3-II.
There are also a few non-specific bands above ~32kD in mouse protein.
Immunohistochemistry (Paraffin): 1:200-1:400 Optimal dilutions to be determined by the researcher.
Positive Control:Human and mouse brain lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 30% glycerol.
Specificity：Recognizes human LC3. Detects both LC3A and LC3B. Species Crossreactivity: mouse, rat, Xenopus, bovine, and zebrafish.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Bo Hu Mo Rt Xe
Immunogen：Synthetic peptide corresponding to an internal portion of the human LC3 protein sequence (between aa25-121). Cellular Localization: LC3-I is cytoplasmic, LC3-II binds to the autophagic membranes
