LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3.
It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development.
However, LC3 is now thought to also be involved in autophagy.
LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction.
LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.
Cellular Localization: LC3-I is cytoplasmic.
LC3-II binds to the autophagic membranes.
Positive Control: Human/mouse brain lysate, ES, HeLa, HEK293, MCF7 cells, human lymphoblasts, and embryoid bodies
Applications:Suitable for use in Western Blot and Immunohistochemistry (paraffin-embedded sections).
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2.
Specificity：This antibody is specific for LC3. Species Crossreactivity: This antibody reacts with human and mouse LC3 protein, though the mouse detection is weaker than the human. The immunogen used for this antibody production has 100% homology with rat and Zebrafish, 92% with mouse, 91% with bovine, and 84% with Xenopus proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt Ze
Immunogen：A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100).
