Versican is a large multi-domain chondroitin sulfate proteoglycan.
It is secreted by fibroblasts and other types of cells.
It has a N-terminal hyaluronic acid (HA) binding domain.
This domain has homology with the G1 and G2 domains of aggrecan (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44.
In addition, Versican also has a lectin-like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan.
Versican has an apparent molecular mass of ~1000kD, of which the core contributes 400kD from the 2409 amino acids, and the 12-15 covalently attached chondroitin sulfate chains make up the rest.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml.
Detects a band at ~70kD
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.6, 1mg/ml BSA, 0.05% sodium azide
Specificity：Recognizes human Versican
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa436-441, CGG-DPEAAE, of human Versican. Cellular Localization: Secreted, extracellular matrix. Species Sequence Homology: mouse and rat
