Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase).
It is absent in resting (G0) cells.
Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms.
In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labelling index.
The correlation between low Ki67 index and histologically low-grade tumors is strong.
Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
Applications:Suitable for use in Immunohistochemistry, Immunocytochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Paraffin): 1:3200Immunofluorescence: 1:50-1:200Immunocytochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Positive Control:Human lymph node
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.09% sodium azide.
Specificity：Recognizes human Ki-67.
Isotype：IgG
Calc Applications Abbrev：IC IF IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to an internal portion of the human protein (within aa1200-1300). [Swiss-Prot# P46013].
