Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase).
It is absent in resting (G0) cells.
Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms.
In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labelling index.
The correlation between low Ki67 index and histologically low-grade tumors is strong.
Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
Applications:Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot, Immunohistochemistry and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Immunofluorescence (IC): 1:10-1:500Western Blot: 1:100-1:2000Immunohistochemistry (Paraffin): 1:100-1:500Immunohistochemistry (Frozen): 1:100-1:500Flow Cytometry: 1:100Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.09% sodium azide.
Specificity：Recognizes mouse Ki-67. Species Crossreactivity: human and rat
Isotype：IgG
Calc Applications Abbrev：FC IC IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to an internal portion (within residues 1600-1700) of mouse Ki-67 protein (Q61769). Cellular Localization: Nuclear
