84-1101-36　［取扱停止］Jagged1 (JAG1, AGS, AHD, AWS, HJ1, JAGL1, Alagille Syndrome Protein, CD339, Headturner, Htu, MGC104644, Serrate-1, Ser-1, Slalom) 500ug　J0400-01F

特徴

• The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development.
• Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions.
• Jagged-1 (JAG1) is a cell surface protein which is one of several ligands that activate Notch and related receptors.
• JAG1 functions in an embryologically important signaling pathway and has also been shown to play a role in hematopoiesis.
• JAG1 inhibits proliferation of cd34+ macrophage progenitor cells and a JAG1 gene abnormality may be an aggravating factor in extrahepatic biliary atresia.
• Activation of NOTCH1 signaling by JAG1 induces monocyte-derived dendritic cell maturation in vitro.
• JAG1 interaction with NOTCH1 on tumor cells dramatically induces proliferation and inhibition of apoptosis in vitro.
• Either haploinsufficiency for wild-type JAG1 and/or dominant negative effects produced by mutated JAG1 are responsible for the Alagille syndrome.
• Applications:Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:20,000-1:100,000Immunofluorescence (IC): 1:200-1:1000Immunohistochemistry: 1:100-1:500Immunohistochemistry (paraffin): 1:10-1:500Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.