IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator.
It is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities.
Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER.
Applications:Suitable for use in Western Blot, ELISA, Immunoprecipitation, Immunofluorescence, Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:1000ELISA: 1:100-1:2000Immunoprecipitation: 1:10-1:500Immunofluorescence (IC): 1:10-1:500Immunohistochemistry (Frozen/Parafin): 1:10-1:500Optimal dilutions to be determined by the researcher.
Positive Control:Glucose treated pancreatic islets and endogenous protein of pancreatic beta cells (20ug) and insuli
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, 0.025% sodium azide
Specificity：Recognizes human IRE1p, phosphorylated (Ser724) at~110kD. Species Crossreactivity: mouse, rat, porcine, Drosophila, goat, mammal, monkey, primate, rabbit and Golden Syrian hamster
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC IP WB
Calc Crossreactivity：Dr Gt Hm Hu Ma Mo Po Rb Rt
Immunogen：Synthetic peptide surrounding the phosphorylated serine 724 of the human IRE1 alpha protein. Cellular Localization: Endoplasmic reticulum
