INHAT (inhibitor of acetyltransferases) complex binds to histones and masks them from being substrate for acetyltransferase.
Endogenous INHAT subunits, which include the Set/TAF-Ibeta oncoprotein, associate with chromatin in vivo and can block coactivator mediated transcription when transfected in cells.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000, a 31kD band is observed in HeLa cellsOptimal dilutions to be determined by the researcher.
Positive Control: HeLa Whole Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in PBS, 0.1mg/ml BSA, 0.02% sodium azide.
Specificity:Recognizes human INHAT-2. Species Crossreactivity: chimpanzee, bovine and rhesus monkey.
Isotype:IgG
Calc Applications Abbrev:WB
Calc Crossreactivity:Bo Hu Mk
Immunogen:Synthetic peptides corresponding to amino acids 87-103 and 231-246 of human INHAT-2. Species sequence homology: chicken (90%), mouse and rat (80%)