Diverse signal transduction pathways impinging on the N-terminal tails of histones lead to a number of post-translational modifications including acetylation, phosphorylation, poly(ADP-ribosylation), ubiquitination and methylation.
These modifications play critical roles in regulating chromatin structure and gene expression.
Histone methyltransferases (HMTases) selectively methylate evolutionarily conserved arginine or lysine residues, primarily in the N-terminal tails of histones H3 and H4.
Set7/9 is a histone specific HMTase that methylates histone H3 lysine 4.
Set7/9 transfers methyl groups to lysine 4 of histone H3 in complex with S-adenosyl-L-methionine.
Human Set7/9 is a 336 amino acid protein.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Specific for Set7/9 (a histone H3 lysine 4 methyltransferase) and does not recognize PR-Set7 (a histone H4 lysine 20 methyltransferase). Species Crossreactivity: Mouse, Human
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Two synthethic peptides corresponding to amino acids 131-145 ([BLAST]GEVNEDGEMTGEKIA[/BLAST]) and 336-352 ([BLAST]GYDHSPPGKSGPEAPEW[/BLAST]) of human Set7/9 were used as immunogen; GenBank Accession No. NP_085151.1. These sequences are 100% conserved between human, mouse and rat.
