84-1101-08　［受注停止］Histone H2A.X, gamma, phosphorylated (Ser139) (H2A Histone Family Member X, H2A.FX, H2A/X, H2AFX, H2AX) [KO Validated] 100ul　H5110-08V4

特徴

• Histone H2AX is a 14kD ubiquitous member of the H2A histone family.
• It contains an evolutionary conserved SQ motif at the C-terminus in eukaryotes.
• Serine 139 within this motif becomes rapidly phosphorylated to yield a form known as gamma-H2AX in response to double-strand DNA damage.
• The response of eukaryotic cells to double-strand breaks in genomic DNA includes the sequestration of many factors into nuclear foci.
• H2AX becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites.
• Applications:Suitable for use in Immunofluorescence, Immunohistochemistry, Flow Cytometry and Western Blot.
• Not recommended for use in Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Immunofluorescence (IC): 1:500-1:5000Immunohistochemistry (frozen): 1:1000-1:5000.
• Immunohistochemistry (Paraffin): 1:1000-1:5000.
• Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Formaldehyde fixation followed by permeabilization with Triton-X 100
• Flow Cytometry: 5ug/1e6 cells in a 150ul reactionWestern Blot: 1:2000-1:10,000Optimal dilutions to be determined by the researcher.
• Positive Control:Human HEK293 nuclear extracts, breast carcinoma, HeLa cells, ovarian carcinoma, testicular seminoma.
• Storage and Stability:May be stored at 4°C.
• For long-term storage, aliquot and store at 4°C.
• Do not freeze.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Further dilutions can be made in assay buffer.