Factor Inhibiting HIF1 (FIH) protein facilitates repression of HIF-1 transcriptional activity by binding to VHL, which acts as a transcriptional corepressor.
VHL inhibits HIF-1 alpha transactivation function by recruiting histone deacetylases.
Involvement of VHL in association with FIH provides a unifying mechanism for the modulation of HIF-1 alpha protein stabilization and transcriptional activation in response to changes in cellular oxygen concentration.
Positive Control: Rat astrocytes and C6 and U251 gliomas
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Tris-citrate/phosphate [pH 7-8] buffer.
Specificity：This antibody is specific for FIH-1 protein. Species Crossreactivity: Human. The immunogen used for this antibody production has 100% homology with the human protein. Other species not tested.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：A C-terminal region synthetic peptide made to the human FIH protein sequence (between residues 300 and the C-terminus).
