GAPDH is a 146kD tetramer composed of four 30-40kD subunits.
Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate.
Because GAPDH as a protein expressed in large amounts and which is required at all times for an important house keeping functions, levels of GAPDH mRNA are often used as standards in studies of mRNA expression.
Increasingly, scientists are making use of specific antibodies to GAPDH as loading controls for western blotting experiments.
Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription.
GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease.
Associations with actin and tubulin have also be reported.
The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.
Applications:Suitable for use in Western Blot.
Not for use in Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in TBS, pH 7.2, 0.1% BSA.
Specificity：Recognizes human Glyceraldehyde 3-Phosphate Dehydrogenase. Species Crossreactivity: mouse. Other species have not been tested. Reactivity with X. laevis and S. Pombe and S. cerevisae GAPDH is expected owing to conservation of sequence between this ortholog and human GAPDH at the site to which the epitope maps.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：The epitope recognized maps to a region between residues 200 and 250 of human Glyceraldehyde-3-Phosphate Dehydrogenase using the numbering given in entry NP_002037.2 (GeneID 2597).
