84-1100-82　Glucose Regulated Protein 78 (Grp78, BiP, GRP 78) 100ug　G3057-20A

特徴

• Within the ER misfolded proteins are detected by Bip (GPR78).
• In the presence of misfolded proteins, Bip disscociates from PERK, allowing it to homodimerize and autophosphorylate.
• In its dimerized, phosphorylated form PERK phosphorylates eIF2.
• The phosphorylation of eIF2 blocks the majority of translation to prevent the continued accumulation of protein in the ER.
• Bip also is binds to IRE1 and ATF6 under normal ER conditions, but dissociates upon accumulation of misfolded proteins.
• Unbound ATF6 is translocated to the gogli where it is converted into a cleaved, active form.
• The cleaved form of ATF6 is then able to up-regulate transcription of UPR genes including XBP1.
• Activated IRE1 acts as an endoribonuclease to an intron from XBP1 transcripts.
• The XBP1 splice variant codes for an active transcription factor which activates transcription of P58IPK.
• P58IPK is a HSP40 protein which binds to and inhibits PERK.
• Thus, if the accumulated, misfolded proteins have been removed, P58IPK acts to shut down the UPR by removing the block to translation.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 2ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Rat liver extract
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.