Methylation of cytosine at CpG dinucleotides is a common feature of many higher eukaryotic genomes.
Increasing evidence indicates that recruitement of histone deacetylase complexes by methyl-CpG-binding proteins (MeCP) is a major mechanism of methylated DNA silencing.
P66, a zinc finger-containing protein is one of the components of the MeCP1 complex.
P66 and p68, the two components of MeCP1 are different forms of the same zinc finger-containing protein and are conserved from C.elegans to humans.
Homologs of p66 from different organisms revealed two highly conserved regions, CR1 and CR2.
While CR1 is involved in the association of p66 with other MeCP1 components, CR2 plays an important role in targeting p66 and MBD3 to specific loci.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry (paraffin): 1:50Optimal dilutions to be determined by the researcher.
Positive Control:p66 alpha Knockout HeLa Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.05% sodium azide.
Specificity：Recognizes mouse p66a. Species Crossreactivity: human and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa137-150 of mouse p66a.
