84-1100-71　Shootin1 100ul　S1013-24

特徴

• The differentiation process of neurons can be divided into five stages, each stage characterized by morphological changes observed in the developing cells.
• In stage 1, the cells extend lamellipodia and in stage 2 their lamellipodia develop into immature neurites.
• In stage 3 one neurite elongates rapidly to form an axon and in stage 4 the remaining immature neuritis elongate to form dendrites.
• In stage 5 synaptic contacts are formed and a neuronal network is established (1).
• Shootin1 is involved in generating internal asymmetric signals required for neuronal during stages 2 and 3.
• The extension of an axon requires considerable reorganization of the cytoskeleton mediated by PI3K/Akt and PI3K/Cdc42 signaling (1).
• Shootin1 is involved in regulating the subcellular localization of PI3 kinase.
• Furthermore, shootin1 is upregulated during polarization and accumulates asymmetrically in a single neurite that consequently elongates rapidly to form an axon (2).
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight
• Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.