The methylation state of lysine residues in histone proteins is a major determinant for formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2).
Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3).
The JmjC domain can catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and alpha-ketoglutarate (3).
Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into 7 separate families (3).
The JARID (Jumonji/AT-rich interactive domain-containing protein) family contains four members: JARID1A (also RBP2 and RBBP2), JARID1B (also PLU-1), JARID1C (also SMCX) and JARID1D (also SMCY) (4).
In addition to the JmJC domain, these proteins contain JmJN, BRIGHT, C5HC2 zinc-finger, and PHD domains, the latter of which binds to methylated histone H3 (Lys9) (4).
All four JARID proteins demethylate di- and tri-methyl histone H3 Lys4; JARID1B also demethylates mono-methyl histone H3 Lys4 (5-7).
JARID1A is a critical RB-interacting protein and is required for Polycomb- Repressive Complex 2 (PRC2)-mediated transcriptional repression during ES cell differentiation (8).
A JARID1A-NUP98 gene fusion is associated with myeloid leukemia (9).
JARID1B, which interacts with many proteins including c-Myc and HDAC4, may play a role in cell fate decisions by blocking terminal differentiation (10-12).
JARID1B is over-expressed in many breast cancers and may act by repressing multiple tumor suppressor genes including BRCA1 and HOXA5 (13,14).
JARID1C has been found in a complex with HDAC1, HDAC2, G9a and REST, which binds to and represses REST target genes in non-neuronal cells (7).
JARID1C mutations are associated with X-linked mental retardation and epilepsy (15,16).
JARID1D is largely uncharacterized.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:25 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：20ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol
Specificity：Recognizes endogenous levels of human JARID1B protein. Does not cross-react with other JARID proteins, including JARID1A, JARID1C and JARID1D. Species Crossreactivity: monkey.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mk
Immunogen：Synthetic peptide corresponding to the human JARID1B protein.
