Inositol 1,4,5-triphosphate receptor, also known as IP3R or InsP3R, is a member of the intracellular calcium release channel family, and is located in the endo- plasmic reticulum.
It functions as a Ca2+ release channel for intracellular stores of calcium ions.
There are three types of IP3 receptors (IP3 receptor 1, 2 and 3) that require the second messenger inositol 1,4,5-triphosphate (IP3) for activation (1).
Four individual receptor subunits, resulting in homo- or hetero-oligomerization of the receptor isoform, form a functional channel (2).
Phosphorylation of IP3R1 at Ser1756 by cyclic-AMP-dependent protein kinase A (PKA) regulates the sensitivity of IP3R1 to IP3 and may thus be a mode of regulation for Ca2+ release (3,4).
IP3R1 mediated calcium release appears to have an effect on the induction of long term depression (LTD) in Purkinje cells (5).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of rat IP3 Receptor only when phosphorylated at Ser1756. Species crossreactivity: mouse and human.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Ser1756 of rat IP3 receptor.
