84-1100-60　Death-associated protein kinase (DAPK1) 100ul　D1051-02

特徴

• Death-associated protein kinase (DAPK1) is a Ca2+/calmodulin-regulated serine/threonine kinase that participates in a wide range of apoptotic signals including interferon-gamma, tumor necrosis factor alpha, Fas, activated c-Myc, and detachment from the extracellular matrix.
• In addition to the kinase domain and calmodulin regulatory segment, DAPK1 also has eight ankyrin repeats, a cytoskeleton binding region, and a conserved death domain (1-3).
• Deletion of the calmodulin-regulatory domain generates a constitutively active mutant kinase.
• Ectopic expression of wild-type DAPK1 induced cell death in HeLa cells.
• Conversely, expression of a catalytically inactive mutant protected cells from interferon-gamma-induced cell death (4).
• The catalytic domain of DAPK1 has very high sequence similarity to vertebrate myosin light chain kinase (MLCK) and a RXX(S/T)X motif derived from myosin light chain protein was shown to be phosphorylated in vitro by DAPK1 (5).
• Epigenetic silencing of DAPK1 by promoter methylation has been observed in cases of chronic lymphocytic leukemia (6,7).
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnightOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.