Endoglin (ENG, CD105) is an auxiliary receptor for the TGF-beta receptor complex, functioning in related signaling pathways (1,2).
Endoglin is a transmembrane protein that exists as a disulfide-linked homodimer.
It is mainly expressed in vascular and connective tissues and in endothelial and stromal cells.
Upregulated endoglin expression has been reported in wound healing, tumor vascularization, inflammatory tissues and in developing embryos (1-4).
Mutations in endoglin have been found to be a causal factor of hereditary hemorrhagic telangiectasia (HHT), a disease characterized by malformation of vascular structure (5,6).
The importance of this protein for normal and tumor vascular function makes it a good marker for endothelial cell proliferation as well as a potential therapeutic target in cancer (4-6).
Application:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes transfected levels of mouse endoglin.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to residues surrounding Pro430 of mouse endoglin.
