84-1100-55　CD95 (Fas) 100ul　C2442-21E

特徴

• Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development and progression of cancers (1,2).
• Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenophathy and splenomegaly as a result of decreased apoptosis in CD4-CD8-T lymphocytes (3,4).
• FasL (CD95L, Apo-1L) is a type 2 membrane protein of 280aa (runs at ~40kD upon glycosylation) that belongs to the TNF family which also includes TNF-alpha, TRAIL and TWEAK.
• Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8 (5).
• Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to the execution of apoptosis.
• Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells and at immunologically privileged sites (6).
• FasL is also expressed in several tumor types as a mechanism to evade immune surveillance (7).
• Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26kD trimeric soluble form (8,9).
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
• Immunohistochemistry (paraffin): 1:480Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.