Catenin delta-1 (p120 catenin) has an amino- terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten, linked 42 amino-acid repeats.
The carboxy-terminal tail has no known function (1).
Catenin delta-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E-cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion (2).
Catenin delta-1 has both positive and negative effects on cadherin-mediated adhesion (3).
Actin dynamics are also regulated by catenin delta-1, which modulates RhoA, Rac and cdc42 proteins (1).
Analogous to beta-catenin, catenin delta-1 translocates to the nucleus although its role at this location is unclear.
Many studies show that catenin delta-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin delta-1 may function as a tumor suppressor (4).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunofluorescence (IF-IC): 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human catenin delta-1 protein only when phosphorylated at Tyr228. Might cross react with another overexpressed phospho-tyrosine protein.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptides corresponding to residues surrounding Tyr228 of human/mouse catenin delta-1.
