FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals.
FOXP3 is essential for normal immune homeostasis
FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells.
FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000-1:5000 detects a band at ~47.2kDImmunofluorescence (IC): 1:10-1:500Immunohistochemistry: 1:50-1:1000.
Tested on mouse thymus from an influenza-infected mouse or thymus from healthy mouse.
Immunohistochemistry (Paraffin): 1:200-1:1000Immunohistochemistry (Frozen): 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human FOXP3. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IC IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to a region between aa400-431 from the C-terminal region of human FOXP3.
