FOXP3 (a 381aa protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals.
FOXP3 is essential for normal immune homeostasis.
FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, at a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells.
FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.
Applications:Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: Band is seen at ~48kD in FOXP3 transfected and CD25/CD4 positive regulatory T cell lysates
Optimal dilutions to be determined by the researcher.
Positive Control:Mouse CD4+CD25+ T cells
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in Tris-glycine, sodium chloride, 0.05% sodium azide.
Specificity：Recognizes mouse FOXP3 (CD4+CD25+T cells). Does not react with human Jurkat or mouse CD4+CD25-T cell lysates.
Isotype：IgG
Calc Applications Abbrev：FC IC WB
Calc Crossreactivity：Mo
Immunogen：Fusion protein encompassing GST-mouse Foxp3 peptide-Flag. Mouse Foxp3 Gly43 to His100 peptide and Flag Tag were fused at C-terminal of GST protein. Cellular Localization: Nuclear
