Fibroblast growth factors (FGFs) are members of a large family of structurally related polypeptides (17-38kD) that are potent physiological regulators of growth and differentiation in a wide variety of cells of mesodermal, ectodermal and endodermal origin.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000-1:2000; 293T cells were transfected with FGFR3 plasmide.
NBT/BCIP was the substrate.
Band at ~110-120kD
Immunohistochemistry (Formalin-fixed paraffin): 1:250-1:500 using Tyrpsin-digested sections.
Stains human placenta and umbilical cord.
Staining is observed in amniotic epithelium, tubules and squamous epithelium
Optimal dilutions to be determined by the researcher.
Positive Control:Whole cell lysates of transfected 293T (embryonic kidney) cells expressing recombinant human FGFR3.
Recommended Secondary Antibodies (IHC):I1904-40N: IgG, Fc, X-Adsorbed (Biotin) Pab Gt x RbI1904-40B: IgG, H&L, X-Adsorbed (AP) Pab Gt x Rb
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 1% BSA, 15mM sodium azide.
Specificity：Recognizes human Fibroblast Growth Factor Receptor 3 (FGFR3) at ~110kD (doublet). Does not react with human FGFR1 or FGFR2.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa792-806 (K-DLLPPAPPSSGG- SRT) of the cytoplasmic region of human FGFR3 with N-terminal added lysine (KLH, glutaraldehyde). Cellular Localization: Plasma membrane; Single-pass type I membrane protein
