Fibroblast growth factors (FGFs) are members of a large family of structurally related heparin binding polypeptides (17-38kD) that are potent physiological regulators of growth and differentiation for a wide variety of cells of mesodermal, ectodermal and endodermal origin.
FGFs are substantially involved in normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, in hematopoiesis as well as in tissue remodeling and maintenance.
They have also been implicated in pathological conditions such as tumorigenesis and metastasis.
The FGF family consists of at least seventeen members designated FGF1 through FGF17.
To date, four genes encoding for high affinity cell surface FGF receptors (FGFRs) have been identified: FGFR1 [flg, cek-1], FGFR2 [bek, cek-3], FGFR3 [cek-2] and FGFR4.
Multiple additional variants (isoforms) arising by alternative splicing have been reported.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Immunoprecipitation: Using whole lysate of transfected cells expressing recombinant human FGFR2
Western Blot: 0.25-0.5ug/ml.
Detects two bands at 115 and 125kD
Optimal dilutions to be determined by the researcher.
Recommended Positive Control:293T, embryonic kidney cell line
Recommended Tissues:Human umbilical cord, rat kidney and mouse tongue
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 1% BSA, 15mM sodium azide.
Specificity：Recognizes human Fibroblast Growth Factor Receptor 2. A doublet at 115-125kD is seen with whole cell lysates of transfected 293T cells, a human, embryonic kidney cell line, expressing recombinant human FGFR2. No reaction with human FGFR1 and FGFR3 is detected.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa809-821, KLPQYPHIGSVKT of the cytoplasmic region of human FGFR2 (KLH by a Glutaraldehyde linker). Species sequence homology: mouse and rat, 92%.
