Fibroblast growth factors (FGFs) are members of a large family of structurally related polypeptides (17-38kD) that are potent physiological regulators of growth and differentiation of a wide variety of cells of mesodermal, ectodermal and endodermal orig
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 1:100 using immunoperoxidase staining of formalin-fixed, paraffin-embedded human and animal sections.
Antigen retrieval: trypsin digestion
Western Blot: 1:400 lysate derived from 293T cells transiently transfected wtih the pcDNA3/FGFR-1 plasmid
Note: The epitopes recognized by the antibody are resistant to routine formalin-fixation and paraffin embedding, and to other fixatives e.g.
Methacarn, Bouins solution, ethanol,and B5
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide
Specificity：Recognizes human FGFR1. Does not react with human FGFR2 or FGFR3. Species Sequence Homology (100%): Mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa360-373, EALEERPAVMTSPLK of human FGFR1 with a C-terminally added lysine (KLH). Cellular Localization: Cell Membrane.
